The human proteome offers deep insights into health, diseases and treatment. Current protein detection techniques are either insensitive, complex or introduce biases, whereas the 20 amino acids have been detected at single-molecule level by surface enhanced Raman spectroscopy (SERS). The RamanProSeq (Grant Agreement ID: 101257819) project aims to develop a label-free SERS-based single-molecule protein sequencing technology capable of reading protein primary sequence at single-amino-acid resolution with high accuracy by integrating solid-state nanopores with a high-speed Raman detection system, an automated control system, computer simulations, and advanced Raman-based bioinformatics. The proposed technology will revolutionise the field of proteomics towards personalized medicine and early diagnosis of diseases.